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Table 5 The values of melting temperature (°C) with standard deviations gaining form high resolution melting (HRM) analysis using matK, rbcL, rpoC and trnL primers of T. laurifolia and C. spectabilis species

From: Fast and reliable detection of toxic Crotalaria spectabilis Roth. in Thunbergia laurifolia Lindl. herbal products using DNA barcoding coupled with HRM analysis

Species

Abbreviation

Tm (oC)

mat K

rbc L

rpo C

trn L

T. laurifolia

T1

-

82.5 ± 0.07

80.2 ± 0.12

78.8 ± 0.07

T. laurifolia

T2

-

82.4 ± 0.14

80.1 ± 0.15

78.9 ± 0.07

T. laurifolia

T3

77.9 ± 0.35

82.3 ± 0.14

80.2 ± 0.00

78.8 ± 0.00

T. laurifolia

T4

-

82.4 ± 0.07

80.2 ± 0.07

78.8 ± 0.14

C. spectabilis

C1

75.6 ± 0.21

81.6 ± 0.00

80.9 ± 0.06

78.4 ± 0.14

C. spectabilis

C2

75.3 ± 0.07

81.6 ± 0.07

80.9 ± 0.06

-

C. spectabilis

C3

75.4 ± 0.21

81.5 ± 0.00

80.8 ± 0.00

78.2 ± 0.00

  1. (-) No amplicons were generated