Fast and reliable detection of toxic Crotalaria spectabilis Roth. in Thunbergia laurifolia Lindl. herbal products using DNA barcoding coupled with HRM analysis

Table 2 Four primers used for HRM analysis and identification

Primer name	Nucleotide sequence (5’ to 3’)	T_a (°C)	Expected size (bp)
matK _F	CTTCTTATTTACGATTAACATCTTCT	57	160
matK _R	TTTCCTTGATATCGAACATAATG
rbcL_F	GGTACATGGACAACTGTGTGGA	57	150
rbcL _R	ACAGAACCTTCTTCAAAAAGGTCTA
rpoC_F	CCSATTGTATGGGAAATACTT	57	170
rpoC _R	CTTACAAACTAATGGATGTAA
trnL_F	GAATCGACCGTTCAAGTATCC	57	150
trnL _R	TATAGGAAACCCATATTTGATCCAATC

ISSN: 2662-7671