Fig. 5

LPE exerts neuroprotective effects on H₂O₂-treated SH-SY5Y cells by modulating the p38 MAPK signaling pathway. a Cells were pretreated with LPE for 6 h, and then co-treated with LPE and 100 μM H₂O₂ for 24 h. Western blotting results demonstrated that H₂O₂ treatment activated phosphorylated (p)-p38; however, LPE pretreatment attenuated the p-p38 level. β-Actin was used as a protein loading control. A representative blot is shown from three independent experiments that yielded similar results. Quantification of band densities for phosphorylated p38/total p38 was measured. Data are expressed as means  ±  SE (n  =  3). **p  <  0.01, compared to vehicle without H₂O₂, ^##p  <  0.01, compared to vehicle with H₂O₂b Cells were pretreated with SB203580 (p38 MAPK inhibitor, 5 μM) for 30 min, 50 μg/ml LPE for 6 h, 100 μM H₂O₂ for 24 h, and then subjected to the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Values are shown as means  ±  SE (n  =  8). **p  <  0.01, compared to vehicle without H₂O₂, ^##p  <  0.01, compared to vehicle with H₂O₂, ††p  <  0.01, compared to H₂O₂ with inhibitor.