Fig. 3

Effects of LPE on H₂O₂-induced cell death in SH-SY5Y cells. a Cells treated with vehicle, H₂O₂ or H₂O₂ + LPE (5 or 50 μg/ml) for 24 h were subjected to flow cytometry analysis after PI and Annexin-V staining. H₂O₂ treatment alone increased late apoptotic cells (R4, upper right quadrant) and necrotic cells (R5, upper left quadrant), but pretreatment of LPE protected against H₂O₂-induced cell death. b Quantitative data showed that percentage of healthy, early apoptotic, late apoptotic, and necrotic cells according to treatment. Values are reported as means  ±  SE (n  =  3). **p  <  0.01, compared to vehicle without H₂O₂, ^##p  <  0.01, compared to vehicle with H₂O₂