Binding signals of GWE-herbochip probed by β-TNF-α. (A) The image was visualized by Cy5-labeled streptavidin after the binding of β-TNF-α to the GWE-herbochip as described in Methods. (B) The fluorescence intensity of the image was quantized by a scanner at 635 nm presented (───) together with the original HPLC profile monitored at 254 nm (− − −−). The controls spots in (A) are 4, 10, 50, 250 ng/mL biotin in Optifix I, 1 μg/mL SA-Cy5 in Optifix II (positive control) and Optifix I (negative control) were spotted onto the slide in the same format shown in Figure 1. Positive signals indicated binding activity of the fractions to TNF-α.