Cell surface marker and cytokine expression of polarized macrophages. CD14, CD11b, HLA-DR, CD40, CD206, CD36 cell surface expression and IL-6 and TNF-α release were measured by flow cytometry or immunoanalysis after M0, M1 or M2 polarization of differentiated macrophages or polarization by co-culture with MCF-7 or NCI-H460 tumour cell lines. Mean results of 5 independent experiments are shown. Data represent the relative MFI (arbitrary units, standardized to experimental mean = 100). Columns with distinct letters differ significantly (p < 0.05) from each other. n.d. = not detectable.