Figure 1
From: Mechanisms of gastroprotection of methanol extract of Melastoma malabathricum leaves
HPLC profile of MEMM at the wavelength 254 nm. A. HPLC analysis of MEMM at wavelength 254 nm shows 7 major peaks that were clearly separated at the retention time (RT) of 14.355, 18.146, 18.575, 18.894, 19.395, 21.047, and 23.657 min. B: Further HPLC analysis was carried out to determine the range of λmax value of the 7 respective peaks detected in MEMM. The chromatogram demonstrates that the λmax value for the six respective peaks falls in the regions of 206.1-279.1, 217.8-273.2, 219.0, 217.8-269.7, 254.3-357.7 and 255.5-349.4 nm, respectively, suggesting, in part the presence of flavonoid-based compounds. Comparison between chromatogram of the standard flavonoid with the chromatogram of MEMM at 300 nm showed the possible presence of quercitrin, which corresponds to peak 7.C: Comparison between HPLC chromatogram of MEMM and quercitrin at 300 nm shows that quercitrin was present in MEMM. The peak that was produced by quercitrin corresponds to peak 7 of MEMM.