Obtained anxiolytic activity in mice performed by climbing out test (A), elevated plus maze-open arm (B), elevated plus maze- close arm (C), forced induced swimming test (D) at 2 hr and 24 hr. NKB produced dose depending activity according to 100, 200 and 400 mg/kg/day. The result of the diazepam (2 mg/kg/day) was used as standard to determine the anxiolytic activity of NKB (p < 0.05). The plant presence in the NKB formulation named Canscora decussata (Roxb.) Schult., Canscora decussata (Roxb.) Schult. And Cannabis sativa Linn. may be the cause of this activity in mice.