Effect of acupuncture at the different acupoints on the expressions of GR and CRH protein in PVN. The vertical ordinate indicated the ratio of GR to β-Actin (the left) and the ratio of CRH to β-Actin (the right). The horizontal ordinate represented the different groups. GR protein expression was obviously reduced in the M group as compare with the N group, while it was dramatically induced in the MEA group as compare with the M group ( #H-300, Santa Cruz, 96KD, dilution: 1:1000). CRH protein expression was obviously increased in the M group as compare with the N and NEA group, while it was obviously reduced in the MEA group as compare with the M group (#10944-1-AP, Datasheet, 36KD, dilution: 1:500).