Table 3 Primers used in RT-PCR
Target gene | Sequence of forward and reverse primers |
|---|---|
NOX4 | 5′-TAGATACCCACCCTCCCG-3′ |
|  | 5′-TGACTGGCTTATTGCTCC-3′ |
p22phox | 5′- GCGGCATCTACCTACTGGC-3′ |
|  | 5′- CTCCTCGCTGGGCTTCTT-3′ |
Rac1 | 5′-GAAGCAGCAGAAATCACAG-3′ |
|  | 5′-CCAATACTCCAGAGGCAAG-3′ |
GTP-Rac1 | 5′-TTAGGGATGATAAAGACACG-3′ |
|  | 5′-GGACAGGACCAAGAACGAG-3′ |
GAPDH | 5′- GGATTTGGTCGTATTGGG-3′ |
|  | 5′- GGAAGATGGTGATGGGATT −3′ |