Figure 3
BYHWD upregulates pVEGFR2 expression and angiogenesis via the PI3K/Akt signaling pathway. A representative immunoblot showed that the effects of ICH, SU5416 and BYHWD on the protein levels of pVEGFR2, VEGFR2, PI3K, pAkt and Akt at the ipsilateral basal ganglion (N and Q). The ratios of pVEGFR2/VEGFR2 (O), pVEGFR2/β-actin, VEGFR2/β-actin (P), PI3K/β-actin (R) and the ratios of pAkt/Akt (S) were compared in ICH and BYHWD with or without SU5416 (mean ± SD, n = 5, * P <0.05, ** P <0.01). Bar = 50 μm. Data were analyzed with Student’s t test and one-way analysis of variance (ANOVA), followed by Scheffe’s post hoc test.