Figure 5
Scanning electron microscopy of mature biofilms fromCandida albicansATCC 10231 treated with fraction F2 or subfraction F2.4 fromStryphnodendron adstringens. Mature biofilms formed during 24-h incubations at 35°C were treated for an additional 24h in the same conditions, with antifungal agent concentrations 64 fold higher than the minimum inhibitory concentration (MIC) values determined for the treatment of planktonic cells. Compared to untreated controls (A-C), mature biofilms treated with 32 μg/mL amphotericin B (D-F) induced a reduction in the fungal biomass. Treatment with 1,000 μg/mL of fraction F2 (G-I) or subfraction F2.4 (J-L) induced the formation of elongated blastoconidia cells (arrows in I and L), and treatment with subfraction F2.4 also induced the formation of blastoconidia clusters (arrow in L). Scale bars: 1 mm (A, D, G and J), 20 μm (B, E, H and K), 10 μm (C, F, I and L).