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Table 2 Primers and conditions used for real time PCR reactions

From: In vitro exposure to very low-level laser modifies expression level of extracellular matrix protein RNAs and mitochondria dynamics in mouse embryonic fibroblasts

Gene Acc. N° Sequences (x-y) Conditions
GAPDH M17701 5-GGCAAGTTCAATGGCACAGTCAAG-3 95°C 30s
5-ACATACTCAGCACCAGCATCACC-3 60°C 30s
40 cycle
Laminin α1 NM_008480.2 5-GGGATGAAGAAGCAAAGCAACT-3 95°C 30s
5-CTCCTTTGCAACACTGCTGTC-3 60°C 45 s
40 cycle
Cadherin 1 NM_009864.2 5-CGACCGGAAGTGACTCGAAA-3 95°C 30s
5-AACCACTGCCCTCGTAATCG-3 60°C 45 s
40 cycle
Integrin α5 NM_010577.3 5-CTCTGTGGCTGTGGGTGAAT-3 95°C 30s
5-CGAAGTAGGAGGCCATCTGTT-3 60°C 45 s
40 cycle
Collagen type I α1 NM_007742.3 5-TCAGCTTTGTGGACCTCCG-3 95°C 30s
5-GGACCCTTAGGCCATTGTGT-3 60°C 45 s
40 cycle