Table 2 Primers and conditions used for real time PCR reactions
Gene | Acc. N° | Sequences (x-y) | Conditions |
|---|---|---|---|
GAPDH | M17701 | 5-GGCAAGTTCAATGGCACAGTCAAG-3 | 95°C 30s |
5-ACATACTCAGCACCAGCATCACC-3 | 60°C 30s | ||
40 cycle | |||
Laminin α1 | NM_008480.2 | 5-GGGATGAAGAAGCAAAGCAACT-3 | 95°C 30s |
5-CTCCTTTGCAACACTGCTGTC-3 | 60°C 45 s | ||
40 cycle | |||
Cadherin 1 | NM_009864.2 | 5-CGACCGGAAGTGACTCGAAA-3 | 95°C 30s |
5-AACCACTGCCCTCGTAATCG-3 | 60°C 45 s | ||
40 cycle | |||
Integrin α5 | NM_010577.3 | 5-CTCTGTGGCTGTGGGTGAAT-3 | 95°C 30s |
5-CGAAGTAGGAGGCCATCTGTT-3 | 60°C 45 s | ||
40 cycle | |||
Collagen type I α1 | NM_007742.3 | 5-TCAGCTTTGTGGACCTCCG-3 | 95°C 30s |
5-GGACCCTTAGGCCATTGTGT-3 | 60°C 45 s | ||
40 cycle |