Mitochondria dynamic analysis in MEF under the different experimental conditions. A. Confocal image of Cyt-C-IR fibroblast. The square in a) indicates the sampled area. B-C. Image processing: B) Background subtraction and gaussian smoothing filter were applied using Imaris software (BitPlane); C) Mitochondrial isosurface reconstruction was obtained with 3D analysis software Imaris. D. Quantitative analysis of total Cyt-C-IR isosurface area. There are no differences between unexposed cells and cells exposed to CW mode, while there is an increase in the values of the total isosurfaces of the cells exposed to PW. Statistical analysis: One way ANOVA, Dunnett’s multiple comparison test **p < 0.01. E. Number of CyT-C IR elements with a volume included between 0.04 and 0.1micron^3. There is an increase in the number of mitochondria in cells exposed to PW Mode. Statistical analysis: one way ANOVA, Dunnett’s multiple comparison test *p < 0.05.