Figure 1

The effect ofH. syriacusskin crude extracts and pure compounds on the cell viability of MDA-MB-231 and HBL100 cell lines. MDA-MB-231(A) and HBL100 (B) were treated with 0, 1, 10, 25, 50, or 100 μg/mL crude extracts for 48 h and subjected to MTT assay to analyze the cell viability of MDA-MB-231 and HBL100 cell lines. (A) MDA-MB-231 cells treated with 100 μg/ml HISY-F2 or HISY-F3 showed 20% cell viability compared with DMSO controls (0.1% DMSO). MDA-MB-231 cells treated with 50 μg/mL of HISY-F3 crude extract showed a cell viability of <50% compared with DMSO controls. (B) Cell viability of HBL-100 cells treated with 50 μg/mL of HISY-F2 or HISY-F3 was 20% of DMSO controls, and the cell viability of HBL-100 cells treated with 100 μg/mL of HISY-F2 or HISY-F3 was less than 20% of that of DMSO controls. (C) Cell viability decreased by 5 μg/mL treatment with K01, K02, K03, K04, or K06 compared with DMSO controls; 10 μg/mL of K02 or K06 reduced cell viability to <50% compared with DMSO controls. (D) Cell viability decreased by 1 μg/mL treatment with K01, K02, K03, K04, or K06 compared with DMSO controls; 10 μg/mL of K01, K02, K03, K04 or K06 reduced cell viability to <50% compared with DMSO controls.