Effect of AG extract on the changes of water content in the brain of MCAO-induced ischemic rats. After MCAO/reperfusion, Evans blue was refused into the brains through i.p. injection. The brains were isolated, and measured the blue-positive area (A) by photo and the blue density in brain extract by spectrophotometry (B). Data in the histogram are expressed as means ± SD of three independent experiments (n = 3 per group). ***P < 0.001 vs. sham; and ###P < 0.001 vs. vehicle.