Effect of AG extract on the expression of ZO-1 and Occludin in the brain of MCAO-induced ischemic rats. The expression of ZO-1 and Occludin was detected in MCAO-induced ischemic brain by western blot (A). β-actin was used as a control. Relative folds of ZO-1 (B) and Occludin (C) were calculated by normalization to β-actin. Data in the histogram are expressed as means ± SD of three independent experiments (n = 3 per group). **P < 0.01 vs. sham; and ##P < 0.01, and ###P < 0.001 vs. vehicle.