Effect of AG extract on the expression of Akt, PI3K and ICAM in the brain of MCAO-induced ischemic rats. After MCAO/reperfusion, the protein was isolated from brain tissues, and assayed for expression of phosphorylated- or whole forms of Akt (A), PI3K (B) and ICAM (C) by western blot. β-actin was used as a control. Relative folds of p-Akt and PI3K and ICAM were calculated by normalization to Akt and β-actin, respectively. Data in the histogram are expressed as means ± SD of three independent experiments (n = 3 per group). **P < 0.01, and ***P < 0.001 vs. sham; and #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. vehicle.