Effect of AG extract on the expression of angiogenesis-regulated molecules in the brain of MCAO-induced ischemic rats. After MCAO/reperfusion, the protein was isolated from brain tissues, and detected the expression of Ang-1, Tie-2 (A) and VEGF (B) by western blot. β-actin was used as a control. Relative folds of Ang-1 (C), Tie2 (D) and VEGF (E) were calculated by normalization to β-actin. Data in the histogram are expressed as means ± SD of three independent experiments (n = 3 per group). **P < 0.01 vs. sham; and #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. vehicle.