Effect of inhalation of EOAH on CRF expression in the paraventricular nucleus in mice subjected to the FST. Photographs represent the distribution of CRF-immunoreactive cells in the paraventricular nucleus of normal (A, n = 8), control (B, n = 9), EOAH 0.5 g (C, n = 9) and EOAH 1.0 g (D, n = 9) groups. The number of the CRF immunostained neurons among the groups (E) was analyzed by one-way ANOVA post-hoc Newman-Keuls test. Each value represents the mean ± S.E.M. ###p < 0.001 compared to the normal group. *p < 0.05 and **p < 0.01 compared to the control group. Sections were cut coronally at 30 μm and the scale bar represents 200 μm. Arrowheads indicate CRF immunopositive neurons.