Figure 7
From: (5R)-5-Hydroxytriptolide (LLDT-8) inhibits osteoclastogenesis via RANKL/RANK/OPG signaling pathway
The effect of LLDT-8 on key proteins of the downstream signaling pathways of RANKL. RAW264.7 cells were treated with LLDT-8 (0 and 50 nM, respectively) for 4 hours, then induced by culture medium supplemented with 50 ng/ml RANKL for 0, 10, 30 and 60 min, respectively. The protein levels of p-IκB, p-P38, p-JNK, p-ERK and p-Akt were detected by western blotting. (A) Immunoblots of the key proteins. RANKL significantly increased the levels of phosphorylation of I-κB, P38, JNK, ERK and Akt in a short time. (B) Relative expression of the key proteins. Compared with the control group, LLDT-8 markedly inhibited the expression of p-IκB induced by RANKL. However, it had little effect on the expression of p-P38、p-JNK、p-ERK and p-Akt induced by RANKL. **p < 0.01, ***p < 0.001, compared to the control group (0 nM) at the same time point.