Figure 3

LLDT-8 inhibited secretion of IL-1β, IL-6, IL-21 and IL-23, but promoted the secretion of IL-10 in the supernatants of PBMCs from peripheral blood of RA patients. PBMCs were isolated from peripheral blood of RA patients and cultured in the presence of anti-human CD3 (0.4 μg/ml) and LLDT-8 (0, 12.5, 25 and 50 nM, respectively) for 48 hours in incubator. The cell supernatants were harvested, and the levels of the proteins were detected by ELISA. (A) The levels of IL-1β in the supernatants of the four groups. (B) The levels of IL-6 in the supernatants of the four groups. (C) The levels of IL-21 in the supernatants of the four groups. (D) The levels of IL-23 in the supernatants of the four groups. (E) The levels of IL-10 in the supernatants of the four groups. *p < 0.05, **p < 0.01, ***p < 0.001, compared to the control group (0 nM).