Figure 3

NJM/fractions reduce clonogenic capacity of MDA-MB-231 cells. MDA-MB-231 cells were treated with 0.1% DMSO (negative control), NJM, NJPE, NJDE, NJEA, and doxorubicin (positive control) for 48 h. After 12 days, cells were stained with crystal violet (0.5% in ethanol) and cell colonies (>50 cells/colony) counted. Results are expressed as mean ± SEM. *P < 0.001 compared with negative control. The treatments were carried out in triplicate.