Anti-emetic mechanisms of zingiber officinale against cisplatin induced emesis in the pigeon; behavioral and neurochemical correlates

Table 4 Effect of ZO-ActFr (50 mg/kg) or standard MCP (30 mg/kg) administered 30 mins before cisplatin challenge, on the level of neurotransmitters and their metabolites (ng/mg tissue wet weight) in the brain areas and intestine in pigeons at t = 18 hr of cisplatin administration (n = 6–8)

Treatment	NA	DOPAC	DA	5HIAA	HVA	5HT
Area postrema
Saline	0.610 ± 0.014	0.382 ± 0.111	0.590 ± 0.146	0.158 ± 0.036	0.913 ± 0.095	0.062 ± 0.034
Cisplatin	0.268 ± 0.073	0.026 ± 0.010	7.366 ± 1.500^###	0.188 ± 0.053	0.556 ± 0.114	0.181 ± 0.052^##
MCP 30 mg	0.023 ± 0.005	0.017 ± 0.006	0.025 ± 0.012	0.005 ± 0.001*	0.121 ± 0.063*	0.023 ± 0.001
ZO-ActFr 50 mg	1.304 ± 1.014	0.229 ± 0.081	0.846 ± 0.407***	0.082 ± 0.044	1.513 ± 0.817	0.127 ± 0.071
Brain stem
Saline	0.094 ± 0.022	0.060 ± 0.020	0.175 ± 0.078	0.060 ± 0.021	0.060 ± 0.016	0.010 ± 0.003
Cisplatin	0.067 ± 0.004	0.001 ± 0.000	0.175 ± 0.026	0.034 ± 0.003	0.009 ± 0.001	0.121 ± 0.010^###
MCP 30 mg	0.119 ± 0.033	0.027 ± 0.006	0.044 ± 0.012	0.007 ± 0.001***	0.066 ± 0.031	0.019 ± 0.002
ZO-ActFr 50 mg	0.014 ± 0.008	0.001 ± 0.000	0.080 ± 0.068	0.001 ± 0.000	0.009 ± 0.005	0.002 ± 0.000***
Intestine
Saline	0.194 ± 0.059	0.067 ± 0.020	0.090 ± 0.064	0.076 ± 0.058	0.056 ± 0.025	0.049 ± 0.016
Cisplatin	0.223 ± 0.036	0.005 ± 0.001	0.151 ± 0.042	0.329 ± 0.054	0.060 ± 0.007	0.463 ± 0.098^###
MCP 30 mg	0.138 ± 0.039	0.054 ± 0.025	0.059 ± 0.018	0.097 ± 0.022	0.198 ± 0.102	0.062 ± 0.013
ZO-ActFr 50 mg	0.172 ± 0.064	0.001 ± 0.000	0.485 ± 0.218	0.009 ± 0.006	0.050 ± 0.030	0.017 ± 0.011***

Values significantly different compared to cisplatin control are indicated as *p < 0.05, ***p < 0.001, while Values significantly different compared to basal level are indicated as ^##p < 0.05, ^###p < 0.001 (ANOVA followed by Tukey post hoc analysis).

ISSN: 2662-7671