Figure 1
Cytoprotective effect ofD. reticulataextract on cell viability. Panel (A) is microscopic images (200×) of RINm5F cells whereas Panel (B) is the effect on % cell viability measured by MTT assay. Pre-treatment with D. reticulata extract was performed on alloxan-induced RINm5F cell damage. The IC50 of alloxan (9 mM) was used to induce cell death after pre-treatment with various concentrations of the extract. In panel (A), A is normal control whereas B is cells treated with alloxan alone. The cells in panels C-H were treated with the extract at 50, 100, 150, 200, 250 and 500 μg/ml, respectively, for 23 hours before exposure of alloxan. Values in Panel (B) are expressed as mean ± S.E.M. (n = 3). * p < 0.05 statistically significant difference compared to control. # p < 0.05 statistically significant difference compared to alloxan (ALX).