Figure 3

Mechanism of antitumor activity of alkaloids from BFU. (A), Effects of peimisine on A2780 cells cycle distribution and the apoptosis rate. The cells were treated with peimisine (15 μg/ml) for 0, 24, 48 and 72 h. (a), 0 h; (b), 24 h; (c), 48 h; (d), 72 h. (B), Histopathological examination of tumor tissue in S180 and LLC tumor models treated with TAFU, CTX and Tween 80 solution as Control by HE staining images (magnification: 40× and 100×), and there are larger areas of necrotic region in the high dose of TAFU and CTX groups than that of Control group. (a), tumor tissue in S180 tumor model; (b), tumor tissue in LLC tumor model. (C), Effects of TAFU on CD31 expression in mice treated with TAFU, CTX and Tween 80 solution as Control. Typical images of immunohistochemical staining of CD31 in tumor mice (magnification: 100×), and the positive cells were stained by brown. (a1), tumor tissue in S180 tumor model; (a2) microvessel density (MVD) of tumor tissue in S180 tumor model; (b1), tumor tissue in LLC tumor model; (b2) MVD of tumor tissue in LLC tumor model. (D) Effects of TAFU on caspase-3 expression in mice treated with TAFU, CTX and Tween 80 solution as Control. Typical images of immunohistochemical staining of caspase-3 in tumor mice (magnification: 400×), and the positive cells were stained by brown. (a1), tumor tissue in S180 tumor model; (a2) IOD of caspase-3 expression in tumor tissue of S180 tumor model; (b1), tumor tissue in LLC tumor model; (b2) IOD of caspase-3 expression in tumor tissue of LLC tumor model. All results were expressed as the mean ± S. E. M., significant differences compared with Control group were designated as ^*P < 0.05 and ^**P < 0.01.