GA induces apoptosis. (A) The expression of caspase-3, −8, −9, and PARP were analyzed by Western blotting. HeLa cells were non treated (NT) or treated with 0.3 μg/ml of GA for 24 h. Effect of GA on the expression of (B) Bcl-2 family and (C) DR5 in HeLa cells. HeLa cells were treated with 0.3 μg/ml of GA for the indicated time points and analyzed by Western blotting. The results from representative experiments were expressed relative to the protein level at 0 h after normalization to β-actin signals.