Figure 3

Effect of Oryeongsan on HG-modulated expression of MMP-2, MT-1 MMP and TIMP-2. (A) Western blot data showing expression of TIMP-2 and MT-MMP-1 in rat mesangial cells treated with Oryeongsan under HG concentrations. β-actin protein was used as an internal control. (B) For gelatin zymography measuring MMP-2 activity, collected culture media were run for electrophoresis on 7.5% SDS-PAGE copolymerized with 0.1% gelatin as the substrate. (C) Effect of Oryeongsan on MMP-2 promoter activity in rat mesangial cell. This was followed by harvesting and determining their luciferase, and β-gal activities were determine. The luciferase activities are expresses relative to the fold of HG and are the mean ± S.E. of five independent experiments. **p < 0.01 vs. control; ##p <0.01 vs. HG alone. The bar graphs (mean ± S.E., n = 3) in bottom panel represent quantitative results obtained from a densitometer. Values not sharing a letter are significantly different as P < 0.05.