Figure 6
Effects of an inhibitor of HO-1 on SHME-mediated protection of DNA damage by H 2 O 2 in C2C12 cells. C2C12 cells were pretreated for 1 h with 300 μg/ml SHME and then treated for 6 h with or without 1 mM H2O2 in the absence or presence of 10 μM ZnPP. (A) The comet assay was performed and representative pictures of the comets were taken using a fluorescence microscope at × 200 original magnification. (B) Cell lysates were prepared and subjected to Western blot analysis with a specific antibody against phospho-histone γH2A.X. Actin was used as a loading control. A representative blot from three independent experiments is shown. The numbers represent the average densitometric analyses as compared with actin in, at a minimum, two or three different experiments.