Anti-inflammatory activity of different spots isolated from hexane fraction. Spot H-(2&3) consisted of two partially overlapped spots. Spot H-4 and H-5 showed high anti-inflammatory activity. L-NAME (250 μM) and induced cell represented a positive and negative control respectively. Each histogram represents a mean of three replicates with error bar representing the standard deviation. *Indicates significant difference (P < 0.05) compared to the positive control.