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Figure 6 | BMC Complementary and Alternative Medicine

Figure 6

From: Essential oils purified from Schisandrae semen inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 activation and migration of human aortic smooth muscle cells

Figure 6

Inhibition of iNOS and COX-2 expression by SSeo in TNF-α-treated HASMCs. (A) HASMCs were pretreated with the indicated concentrations of SSeo 1 h prior to incubation with TNF-α (100 ng/ml) for 6 h. Total RNA was prepared for RT-PCR analysis of iNOS and COX-2 gene expression. The reaction products were run on 1% agarose gel electrophoresis and visualized by EtBr staining. (B) After TNF-α treatment for 24 h, cell lysates were prepared and Western blotting was performed using anti-iNOS and anti-COX-2 antibodies, and an ECL detection system. GAPDH and actin were used as the internal controls of RT-PCR and Western blot analysis, respectively.

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