Table 1 Primers sequences for reverse transcription–polymerase chain reaction (RT–PCR)
Gene name | Primer(upper:sense primer) |
|---|---|
(Gene bank) | (Lower: antisense primer) |
Col2a1 | F: 5'-TGAAGACATCCGCAGCCCC -3' |
(NM_031163) | R: 5'-ATAATGGGAAGGCGGGAGG-3' |
MMP-3 | F: 5'-CCTTTTGATGGGCCTGGAA-3' |
(XM_284824) | R: 5'-ATCGTCAAAGTGAGCATC-3' |
MMP-13 | F: 5'-CTTGTGTTTGCAGAGCACTA -3' |
(NM_008607.1) | R: 5'-ACTGTGGAGGTCACTGTAGA-3' |
ADAMTS5 | F: 5'-GCCATTGTAATAACCCTGCACC-3' |
(XM_122978.1) | R: 5'-TCAGTCCCATCCGTAACCTTTG-3' |
EPAS1 | F: 5'-CGAGAAGAACGACGTGGTGTTC-3' |
(NM_010137.2) | R: 5'-GTGAAGGCGGGCAGGCTCC-3' |
β-Actin | F: 5’-CAGTTCGCCATGGATGACGAT3’ |
(NM_007393) | R: 5’-CATAGCTCTTCTCCAGGGAG-3’ |