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Table 1 Primers sequences for reverse transcription–polymerase chain reaction (RT–PCR)

From: Arthropod steroid hormone (20-Hydroxyecdysone) suppresses IL-1β- induced catabolic gene expression in cartilage

Gene name Primer(upper:sense primer)
(Gene bank) (Lower: antisense primer)
Col2a1 F: 5'-TGAAGACATCCGCAGCCCC -3'
(NM_031163) R: 5'-ATAATGGGAAGGCGGGAGG-3'
MMP-3 F: 5'-CCTTTTGATGGGCCTGGAA-3'
(XM_284824) R: 5'-ATCGTCAAAGTGAGCATC-3'
MMP-13 F: 5'-CTTGTGTTTGCAGAGCACTA -3'
(NM_008607.1) R: 5'-ACTGTGGAGGTCACTGTAGA-3'
ADAMTS5 F: 5'-GCCATTGTAATAACCCTGCACC-3'
(XM_122978.1) R: 5'-TCAGTCCCATCCGTAACCTTTG-3'
EPAS1 F: 5'-CGAGAAGAACGACGTGGTGTTC-3'
(NM_010137.2) R: 5'-GTGAAGGCGGGCAGGCTCC-3'
β-Actin F: 5’-CAGTTCGCCATGGATGACGAT3’
(NM_007393) R: 5’-CATAGCTCTTCTCCAGGGAG-3’