Figure 4

Effect of laser light irradiation on mitochondria membrane potential. The figure shows results from mitochondria function experiments. Micrographs A-D illustrate JC-1 accumulation in mitochondria of healthy cells (A, B) and of cells exposed to H₂O₂ (C, D), at the beginning (A, C, t = 120 sec) and end (B, D, t = 960 sec) of the observational period. E, F: graphs represent data from a typical experiment, showing the decrease over the time of fluorescence intensity values over the observational time in the absence (photobleaching due to confocal laser stimulation) and at the presence of H₂O₂ (oxidative stress). G, H: Δ intensity illustrates the effect of a single 20 sec (H) and 15 min (G) laser light irradiation on mitochondria membrane potential during H₂O₂-induced oxidative stress. Both MOD1 and MOD2 exposure to red light radiation prevent MMP variation due to oxidative stress. Statistical analysis: one-way ANOVA and post-hoc test, *p < 0.05.