The timecourse of cytokine concentrations into the media from PBMCs over the first 3 days after stimulation with PHA. PBMCs from a healthy volunteer were exposed to PHA as described in Materials and Methods. Media from the cells was collected 1, 2 and 3 days after stimulation and was assayed for cytokine concentrations using a multiplex bead assay. Data is presented as the percentage of cytokine concentration in cells at the timepoint with maximum cytokine concentration, which in each case was 3 days after PHA stimulation. Data represents the average of three replicates ± standard deviation.