The aqueous extract from Immuplex
® and Congaplex
® alter cytokine RNA expression following activation. CEM (A) and Jurkat (B) cells were pre-treated the aqueous extract from Immuplex® or Congaplex® for one hour prior to treatment with PMA and PHA as described in Materials and Methods. CEM and Jurkat cells were harvested 6 and 3 hours after PMA/PHA treatment, respectively, and cells were assayed for mRNA expression corresponding to cytokines using real-time PCR. Data are representative of 3 independent experiments, using independent cell cultures, all of which produced similar results. Data represents the average of four replicates ± standard deviation. Data is presented as the percentage of cytokine concentration in cells treated with PMA and PHA in the absence of Immuplex® or Congaplex® treatment (vehicle). * = significantly higher than vehicle treatment. † = significantly lower than vehicle treatment.