Figure 8

PPC enhanced the IFNγ ⁺ /CD8 ⁺ T cell response to a dendritic cell vaccine specific for a melanoma antigen. PPC enhanced the dendritic cell vaccine induced production of melanoma associated Trp2-specific IFNγ⁺/CD8⁺ T cells. CD8⁺ T cells were isolated from the splenocytes population and plated with stimulator cells (EL4 only, EL4 pulsed with the Trp2₁₈₀ peptide, or EL4 pulsed with the irrelevant OVA₂₅₇ peptide). Mice receiving no PPC are represented by the open bars while those receiving 200 μg/mL PPC ad libitum are represented by the filled bars. Error bars represent the standard deviation. The delivery of PPC was found to significantly enhance the number Trp2₁₈₀ specific IFNγ⁺/CD8⁺ T cells (p = 0.002 for EL4 Trp2₁₈₀).