Figure 7

PPC enhanced the IFNγ ⁺ /CD8 ⁺ T cell response to a DNA vaccine specific for ovalbumin. PPC enhanced the DNA vaccine induced production of OVA-specific IFNγ⁺/CD8⁺ T cells. Mice were immunized by DNA injection followed immediately by electroporation. Mice were injected with DNA encoding full-length ovalbumin and then the injection area was immediately exposed to 95 volts in four pulses of 65 milliseconds using an Electro Square Porator device (BTX, model TX830). CD8⁺ T cells were isolated from the splenocytes population and plated with stimulator cells (EL4 only, EL4 pulsed with the OVA₂₅₇ peptide, or EG7 cells). Mice receiving no PPC are represented by the open bars while those receiving 200 μg/mL PPC ad libitum are represented by the filled bars. Error bars represent the standard deviation. The delivery of PPC was found to significantly enhance the number OVA specific IFNγ⁺/CD8⁺ T cells (p = 0.009 for EL4 OVA₂₅₇ and p = 0.005 for EG7).