Exposure of SCp2 cells to Y
reduces ET-induced IL-6 levels more potently than fish oil and CLA. SCp2 cells were treated (A and C) on day 3 of culture 30 min before ET (short-term exposure) or (B and D) for 3 days (long-term exposure), as of day 1 of culture, with EtOH (Ctrl), Y2+3
a, fish oil (A and B) or CLAb (C and D) at different concentrations. Cells were treated on day 3 with ET at 10 μg/ml in DM supplemented with 1% FBS and their media collected 9 hr post-ET. The values depicted are the means (± S.D.) of a triplicate treatment. Statistical significance of the difference from Ctrl+ET is with **P < 0.01 or ***P < 0.001. (Ctrl, control; ET, endotoxin; IL-6, interleukin-6). a
Cells were treated with Y
at 5 or 10 μg/ml for long- (B and D) and short-term (A and C) exposure, respectively. b
Short- and long-term exposures to CLA at 5 and 3 μg/ml, respectively, were cytotoxic to cells. -ET (white square); +ET (black square).