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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Interleukin-6 and Cyclooxygenase-2 downregulation by fatty-acid fractions of Ranunculus constantinopolitanus

Figure 4

Exposure of SCp2 cells to individual FA components of Y 2+3 or a synthetic FA mix does not reduce ET-induced IL-6 levels. SCp2 cells were treated (A) on day 3 of culture 30 min before ET (short-term exposure) or (B) for 3 days (long-term exposure), as of day 1 of culture, with EtOH (Ctrl), Y2+3 a, palmitate, linoleateb, oleate, stearate or a synthetic FA mix at different concentrations. Cells were treated on day 3 with ET at 10 μg/ml in DM supplemented with 1% FBS and their media collected 9 hr post-ET. The values depicted are the means (± S.D.) of a triplicate treatment. Statistical significance of the difference from Ctrl+ET is with ***P < 0.001. (Ctrl, control; -ET, untreated cells; IL-6, interleukin-6). a For short-term exposure (A) , cells were treated with Y 2+3 at 10 μg/ml. b Long-term exposure (B) to linoleate at 5 μg/ml was cytotoxic to cells.

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