Exposure of SCp2 cells to individual FA components of Y
or a synthetic FA mix does not reduce ET-induced IL-6 levels. SCp2 cells were treated (A) on day 3 of culture 30 min before ET (short-term exposure) or (B) for 3 days (long-term exposure), as of day 1 of culture, with EtOH (Ctrl), Y2+3
a, palmitate, linoleateb, oleate, stearate or a synthetic FA mix at different concentrations. Cells were treated on day 3 with ET at 10 μg/ml in DM supplemented with 1% FBS and their media collected 9 hr post-ET. The values depicted are the means (± S.D.) of a triplicate treatment. Statistical significance of the difference from Ctrl+ET is with ***P < 0.001. (Ctrl, control; -ET, untreated cells; IL-6, interleukin-6). a
For short-term exposure (A) , cells were treated with Y
at 10 μg/ml. b
Long-term exposure (B) to linoleate at 5 μg/ml was cytotoxic to cells.