Figure 6

(A) Electrophoretic Mobility Shift Assay showing the effect of SA on NF-κB activation in the presence and absence of IL-1 in Mode-K cells. IL-1 treatment for 6 h caused activation of NF-κB (lane 2). This IL-1-induced activation was significantly inhibited by pretreating Mode-K cells with 4 μg/ml SA for 2 h (lane 4). (B) Western blotting assay showing the effect of SA on IκB-α protein expression in the presence and absence of IL-1 in Mode-K cells. IL-1 treatment for 6 h caused degradation of IκB-α protein resulting in decreased protein levels (lane 2). This IL-1-induced decrease was blocked by SA pretreament for 2 h (lane 4). No effect of SA on IκB-α protein levels was observed (lane 3). β-Actin was used to ensure equal protein loading.