Figure 5
From: Anti-tumor effect of Liqi, a traditional Chinese medicine prescription, in tumor bearing mice
Effect of Liqi on TNF-α activity in tumor-bearing mice. Sarcoma 180 tumor cells were subcutaneously implanted (2 × 106 cells suspended in 50 μl of PBS) in the right axillary region of syngeneic BALB/c mice. After 24 h, Liqi (50 g/kg) was administrated daily by gavage for 12 days. Then tumor-bearing BALB/c mice received 20 μg/0.2 ml of LPS intravenously. After 1.5 h, blood samples were collected from the mice's orbital plexuses. TNF activity was determined by a cytotoxicity assay on L929 cells. The percent cytotoxicities were plotted against the logarithm of sample quantity. One unit of TNF activity was defined as the sample quantity required to achieve 50% cytotoxicity in the reaction. Results are expressed as the mean ± S.D. of 12 mice. ** P < 0.01, compared with untreated control of tumor-transplanted animals (model group).