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Table 3 Proteins up regulated by F4 Fraction

From: Petiveria alliacea extracts uses multiple mechanisms to inhibit growth of human and mouse tumoral cells

Up regulated Proteins

Accesion Number

Translational Proteins

 

Alanyl t-RNA synthetase variant

BAD96544

CDA02 (Eukaryotic translation initiation factor 2A)

AAK14926

Eukaryotic translation initiation factor 2, subunit 1 alpha, 35 kDa

NP_001406

Eukaryotic translation initiation factor 3 subunit A, KIAA0139

BAA09488

GA17 protein (eukaryotic translation initiation factor 3, subunit M)

NP_006351

GCN1 general control of amino-acid synthesis 1-like 1, KIAA0219

BAA13209

Leucyl-tRNA synthetase, cytoplasmic, KIAA1352

BAA92590

Methionine adenosyltransferase II, alpha

NP_005902

Mitochondrial isoleucine tRNA synthetase

NP_060530

Prt1 homolog, Eukaryotic translation initiation factor 3 subunit B

AAB42010

Synaptotagmin binding RNA interacting protein, SYNCRIP

AAH24283

Transcription processing

 

DNA-binding protein A (Cold shock domain-containing protein A)

P16989

Heterogeneous nuclear ribonucleoprotein M isoform a

NP_005959

Small nuclear ribonucleoprotein polypeptide F

NP_003086

Small nuclear ribonucleoprotein Sm D1

CAE11897

Ribosomal Proteins

 

Ribosomal protein S15

NP_001004

Ribosomal protein S9

NP_001009

Ribosomal Protein S3A

NP_000996

Protein Degradation

 

Proteasa de Cisteina del Retículo (ER60)

BAA11928

Proteasome 26S ATPase subunit 1 variant

BAD96388

Proteasome 26S ATPase subunit 2

NP_002794

Proteasome 26S non-ATPase subunit 11 variant

BAD96916

Proteasome 26S non-ATPase subunit 2 variant

BAD93080

Putative ubiquitin-conjugating enzyme E2 D3-like protein

Q9NTT1

SUMO1 activating enzyme subunit 1

NP_005491

Tripeptidyl peptidase II

CAH72178

Ubiquitin-Activating enzime E1

NP_003325

Transporters

 

Amino acid transporter E16

AAC61479

ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 isoform 1

NP_733765

Coatomer protein complex subunit alpha isoform 1 (Cop I)

NP_001091868

Exportin 1

NP_003391

Karyopherin beta 1, Importin subunit beta-1

NP_002256

SEC13-like 1 (S. cerevisiae), isoform CRA_b

EAW64078

Signal recognition particle 72 kDa

NP_008878

Solute carrier family 25 (mitochondrial carrier, Aralar), member 12

NP_003696

Stomatin

AAH10703

Cytoskeleton

 

Actin related protein 2/3 complex subunit 2

NP_005722

ARP3 actin-related protein 3 homolog

NP_005712

Chaperonin containing TCP1-subunit 2 beta

EAW97230

Chaperonin containing TCP1-subunit 3 gamma

BAD92119

Destrin, isoform a

NP_006861

Dynactin 1 isoform 1

NP_004073

Dynamin 1-like, isoform CRA_c

EAW88521

Filamin A, FLJ00343

AAF72339

MYO1C variant protein (myosin-I beta)

BAE06097

T-complex protein 1 subunit epsilon, KIAA0098

BAA07894

Transgelin-2, KIAA0120

BAA04802

Cell Cycle

 

Alpha isoform of regulatory subunit A, protein phosphatase 2

NP_055040

Minichromosome maintenance complex component 6

NP_005906

Poly (ADP-ribose) polymerase family, member 1

NP_001609

Regulator of chromosome condensation 1, isoform CRA_c

EAX07692

Septin 9, KIAA0991

BAA76835

Signal Transduction

 

GTP-binding protein PTD004 isoform 1

NP_037473

Phosphofructokinase, platelet, isoform CRA_a

EAW86495

Protein kinase C inhibitor protein 1, YWHAZ

AAH51814

Chaperones

 

Calnexin precursor

NP_001737

Nucleophosmin

AAW67757

Oxygen regulated protein precursor

NP_006380

TNF receptor-associated protein 1 variant

BAD93042

Metabolism

 

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase

NP_004035

Acyl-CoA synthetase long-chain family 3

NP_976251

Aldehyde dehydrogenase 18 family, member A1

CAI16766

Alkylglycerone phosphate synthase, isoform CRA_b

EAX11058

Carbamoylphosphate synthetase 2/aspartate transcarbamylase/dihydroorotase

NP_004332

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase

CAH73476

Enoyl Coenzyme A hydratase

AAH08906

Glucosamine–fructose-6-phosphate aminotransferase (GFAT 1)

Q06210

HMT1 hnRNP methyltransferase-like 2 isoform 1

NP_001527

Human rab GDI

BAA03095

Hydroxyacyl-Coenzyme A dehydrogenase

AAH14572

Inosine monophosphate dehydrogenase 2, hCG2002013

EAW64946

Ornithine aminotransferase precursor

NP_000265

Phosphogluconate dehydrogenase

NP_002622

Phosphoribosyl pyrophosphate synthetase 2, PRPS2

NP_002756

RPN2

CAG33180

S-adenosylhomocysteine hydrolase

NP_000678

  1. Proteins from A375 human cells treated with F4 or ethanol (0.2%) as negative control were digested, and the extracted peptides injected onto a 1100 Series HPLC-Chip Cube MS interface, and Agilent 6300 Series Ion Trap Chip-LC-MS/MS system (Agilent Technologies). Data are the proteins up regulated by F4 fraction treatment as judged by mass spectrometry.