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Table 2 Proteins down regulated by F4 Fraction

From: Petiveria alliacea extracts uses multiple mechanisms to inhibit growth of human and mouse tumoral cells

Down regulated proteins

Accesion Number

Translation

 

Asparaginyl-tRNA synthetase

NP_004530

BAT1 protein

BAF31287

CGI-74 protein

AAD34069

Dhx9 (DEAH) P-9, RNA Helicasa A (RHA)/DEAH (Asp-Glu-Ala-His)

NP_001348

Eukariotic translation elongation factor 1 gamma

AAH13918

Eukariotic translation elongation factor-2 (EF-2).

NP_001952

Eukaryotic initiation factor 4A (eIF-4A)

NP_001407

Heparin-binding protein HBp15 solo un articulo

AAP97261

Heterogeneous nuclear ribonucleoprotein H1

NP_005511

Heterogeneous nuclear ribonucleoprotein U isoform a (scaffold attachment factor-A)

NP_114032

Interleukin enhancer binding factor 3, 90 Kda

EAW84137

Nucleolin

NP_005372

Nucleosome assembly protein 1-like 1

NP_631946

Poly(rC)-binding protein 2 isoform b variant PCBP2

NP_114336

Small nuclear ribonucleoprotein Sm D1

CAE11897

Tryptophanyl-tRNA synthetase (IFP53)

CAA44450

Tu-transcription elongation factor. ET-1 o EF-Tu

NP_003312

Transcription processing

 

CGI-74

AAD34069

DEAD (Asp-Glu-Ala-His) box polypeptide 21

NP_004719

DEAD (Asp-Glu-Ala-His) box polypeptide 9

NP_001348

Ribosomal Proteins

 

Ribosomal protein L4

NP_000959

Ribosomal protein L5

EAW73088

Ribosomal protein L7a

EAW88064

Ribosomal protein L8

NP_000964

Ribosomal protein L9

NP_000652

Ribosomal protein L10

NP_006004

Ribosomal protein L10a

NP_009035

Ribosomal protein L11

NP_000966

Ribosomal protein L12

BAD92708

Ribosomal protein L13

NP_150254

Ribosomal protein L13a

AAQ13495

Ribosomal protein L14

NP_004964

Ribosomal protein L18

NP_000970

Ribosomal protein L18a

XP_943475

Ribosomal protein L19

EAW60568

Ribosomal protein L23

NP_000969

Ribosomal protein L26

NP_001087230

Ribosomal protein L27

NP_000979

Ribosomal protein L27a

NP_001083056

Ribosomal protein L28

NP_000982

Ribosomal protein L31

NP_000984

Ribosomal protein L32

NP_000985

Ribosomal protein L36

NP_378669

Ribosomal protein L37a

NP_000989

Ribosomal protein S2

NP_002943

Ribosomal protein S4

EAW71815

Ribosomal protein S5

BAD93040

Ribosomal protein S6

NP_001001

Ribosomal protein S8

EAX07023

Ribosomal protein S11

NP_001006

Ribosomal protein S12

EAW54624

Ribosomal protein S13

NP_001008

Ribosomal protein S15a

EAW50259

Ribosomal protein S18

NP_001087248

Ribosomal protein S19

NP_001013

Ribosomal protein S24

EAW54624

Ribosomal protein S25

NP_001019

Ribosomal protein S27

EAW91426

Ribosomal protein P0 variant

BAD96291

Replication

 

CSE1 chromosome segregation 1-like protein

NP_001307

ErbB3 (HER3) binding protein 1

NP_006182

Nucleolin

NP_005372

Protein Kinase, DNA-activated, catalytic polypeptide isoform 1

NP_008835

Degradation Proteins

 

26S proteasome subunit p45

BAA07919

Flap structure-specific endonuclease 1

NP_004102

Fumarate hydratase, isoform CRA_b

EAW70091

Histone cluster 1, H1d

NP_005311

HMG-1

BAA09924

Hydroxysteroid (17-beta) dehydrogenase 10 isoform 1

NP_004484

Mitochondrial acetoacetyl-CoA thiolase

BAA01387

PSMC3 protein

AAI07805

Transporters

 

Mitochondrial trifunctional protein, alpha subunit precursor

NP_000173

Prohibitin 2 (Phb2)

NP_009204

Solute carrier family 25 (SLC25A5 protein)

AAH68199

Cytoskeleton

 

Actin, gamma 1 propeptide

NP_001605

Adenylyl cyclase-associated protein

NP_006358

Chaperonin containing TCP-1 (subunit 6 A and 7)

NP_006420

Dynein light chain 1

NP_003737

F-actin capping protein alpha 1

NP_006126

Kinesin

NP_004512

Lamin A/C, isoform CRA_c

AAH00511

Lamin B1

NP_005564

Miller-Dieker lissencephaly protein

AAL34972

Myosin heavy polypeptide 9

NP_002464

Myosin, light polypeptide 6B, alkali, smooth muscle and non-muscle, isoform CRA_c

EAW96898

Plectin

NP_958782

t-complex polypeptide 1 (TCP-1)

CAA37064

Tubulin alpha 6

NP_116093

Signal Transduction

 

RAB5C, member RAS oncogene family isoform b

NP_004574

Guanine nucleotide binding protein (G-protein)

EAW53700

IQ Motif containing GTPase activating protein 1

BAA06123

Prohibitin (PHB)

CAG46507

RAN member RAS oncogene familly

EAW98517

Chaperones

 

HSP70-prot 8

NP_006588

HSP60

ABB01006

Tumor rejection antigen (gp96) or Heat schock protein 90 Kda beta

CAI64497

Heat shock protein 90 kDa alpha (HSP90)

NP_005339

Metabolism

 

ACLY variant protein

BAE06117

ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit precursor

NP_001677

Dihydropyrimidinase-like 2 variant

BAD92432

Enolase 1, variant

BAD96912

Fatty acid synthase

AAA41145

Glucose phosphate isomerase

NP_000166

Glucosidase II

CAA04006

Glyceraldehyde-3-phosphate dehydrogenase

NP_002037

Lactate dehydrogenase A

NP_005557

Peroxiredoxin 6

NP_004896

Phosphoglicerate kinase (PGK)

NP_000282

Phosphoglycerate dehydrogenase

NP_006614

Prostaglandin E synthase 3 (cytosolic)

AAH03005

Pyruvate Kinase, muscle isoform CRA_c

AAH12811

Tumoral Antigen

 

Melanoma-associated antigen 4 (MAGE 4 antigen)

P43358

Calcium binding proteins

 

Annexin 5

NP_001145

Annexine A2 isoform 1

NP_001002858

  1. Proteins from A375 human cells treated with F4 or ethanol (0.2%) as negative control were digested, and the extracted peptides injected onto a 1100 Series HPLC-Chip Cube MS interface, and Agilent 6300 Series Ion Trap Chip-LC-MS/MS system (Agilent Technologies). Data are the proteins down regulated by F4 fraction treatment as judged by mass spectrometry.