Figure 4From: Petiveria alliacea extracts uses multiple mechanisms to inhibit growth of human and mouse tumoral cells F4 fraction has no activity on mitochondrial membrane depolarization. A. K562 human cells were treated with F4 fraction (31.2 μg/ml), or positive control S2 fraction (15.6 μg/ml) or ethanol (0.2%) for 4, 8 and 12 h. All cells were stained with JC-1 (Sigma) dye and analyzed by flow cytometry (FACScalibur CellQuest software program) (Becton Dickinson). Bars represent cell percentage (%) ± SEM of depolarized (filled bars) or non-depolarized (hatched bars) cells, representing two independent experiments. ***p < 0.001 versus control (ethanol 0.2%; Unpaired Student's t-test). B. A375 cells treated with ethanol (0.2%) (left panel) or F4 fraction (31.2 μg/ml) (right panel) for 24 h were permeabilized, stained with DAPI and analyzed under fluorescence microscope (Olympus). Results show photos representing four independent experiments.Back to article page