Figure 3

A, B. Detection of apoptosis by TUNEL assay in human primary osteoclasts. The presence of apoptotic OCs after treatment with 0.5, 5, 50 μg/ml of Emblica officinalis extract for 60 hours is shown in panel A. Brown color reaction indicates cells that underwent apoptosis. Quantitative results of the experiment shown in panel A are depicted in panel B (the data reported represent the average ± SD of six independent determinations). C. Immunocytochemical analysis of FAS receptor expression levels in human primary osteoclasts subjected to the same experimental conditions reported in panel A. (-): control cells. Cells were photographed at the 20 × magnification.