Figure 4

Effects of Kihi-to on Aβ(25–35)-induced decreases in dendrites. Aβ(25–35) (25 nmol) was injected into the right lateral ventricle of mice. From ten days after the injection, mice were administered vehicle (V, water by p.o.; DMSO by i.v.) or Kihi-to (K, 100 mg/kg B.W., p.o.) for 3 days. The control mice (C) were injected with a reverse peptide, Aβ(35–25) and then administered vehicle. After the novel object recognition test (Figure 2), brain slices were immunostained with MAP2 antibody. MAP2-positive areas were quantified in the stratum oriens and stratum radiatum in CA1, the stratum oriens, stratum radiatum and stratum lucidum in CA3, the molecular layer and hilus in the dentate gyrus (DG), and the parietal, perirhinal and frontal cortex. *p < 0.05 vs. Veh. n = 3. (one-way ANOVA followed by Holm-Sidak post hoc test).