Figure 13

Effects of Kihi-to on Aβ(25–35)-induced Ca ²⁺ influx. Cortical neurons were cultured for 7 – 8 days and then loaded by fluo-4 AM (8 μM) for 40 min. After additional incubation for 30 min, cells were stimulated by 10 μM Aβ(25–35) alone (b; circles, n = 125) or 10 μM Aβ(25–35) and 1 μg/ml Kihi-to (b; squares, n = 83). Time-lapse images were captured every 5 s. Peak fluorescence change was calculated as relative change from baseline (ΔF/F%). Typical images were shown in (a). Scale bar = 10 μm.