Table 4 Log probit analysis of the larvicidal activity of chloroform:methanol extract of the green berries of S. villosum on different instar larvae of S. aegypti. LC, lethal concentration; R, coefficient of regression equations.
Type of instars of mosquito larvae | Period of bioassay (hours) | Regression equations | R 2 | LC50 values (ppm) | Lower and upper fiducidal limits (ppm) |
|---|---|---|---|---|---|
First | 24 | Y = 29.82 + 0.820x | 0.97 | 22.06 | 16.05–27.66 |
|  | 48 | Y = 33.16 + 0.820x | 0.97 | 19.58 | 14.36–24.20 |
|  | 72 | Y = 31.19 + 0.923x | 0.98 | 19.19 | 13.97–23.45 |
Third | 24 | Y = 29.83 + 0.820x | 0.97 | 11.67 | 8.49–14.84 |
|  | 48 | Y = 33.16 + 0.820x | 0.97 | 9.54 | 6.82–12.25 |
|  | 72 | Y = 31.19 + 0.923x | 0.98 | 5.97 | 2.15–9.79 |
Fourth | 24 | Y = 25.98 + 0.282x | 0.99 | 49.84 | 44.53–54.77 |
|  | 48 | Y = 31.19 + 0.256x | 0.82 | 21.22 | 13.30–29.13 |
|  | 72 | Y = 34.53 + 0.256x | 0.82 | 21.02 | 15.97–73.94 |