Figure 5
From: Novel phytoandrogens and lipidic augmenters from Eucommia ulmoides
Radioligand displacement assay of E. ulmoides (EU) extract. Tritiated testosterone was incubated with transfected Hela cells transiently expressing AR protein. Concentration-dependent competitive displacement of tritiated testosterone by androgenic E. ulmoides (EU) extract (1c.f. = 50 ng/ml treatment medium) was measured by the scintillation count of the bound radioisotopic testosterone to AR protein. DHT (nM) and cortisol (nM) acted as positive and negative controls respectively. Data are mean ± SE of four replicates.