Figure 2

PAC-induced effects on cell proliferation in vitro. 96-well assay plates with media containing 10% fetal bovine serum (FBS) in the absence and presence of increasing PAC dosages [0–100 μg/mL] and cells were allowed to proliferate for four days. The addition of PAC at low concentration (10 μg/mL) stimulated proliferation of Ca Ski (A), CAL 27 (E) and CAL 27-TF16 (G) cells, but inhibited proliferation of GH354 (C) and Hs27 (I) cells. Measurement of the relative change in proliferation between day 4 and day 1 revealed that the maximal PAC growth inhibition dose (GI_MAX) was between 30–60 g/mL for all cell lines tested (B,D,F,H,J).